⚕️ URINALYSIS: MANUAL STEP-BY-STEP GUIDE
πΉ STEP 1: PATIENT PREPARATION AND SAMPLE COLLECTION
Explanation:
A clean-catch midstream urine sample is best to avoid contamination from skin or genital secretions.
Steps:
1. Instruct the patient to clean the genital area with clean water or antiseptic wipes.
2. Ask the patient to void the first part of urine into the toilet.
3. Collect the midstream portion in a clean, dry, sterile container.
4. Close the lid tightly and label the container with the patient's details and time of collection.
πΉ STEP 2: PHYSICAL EXAMINATION (MACROSCOPIC)
Explanation:
Observes the urine’s appearance to detect abnormalities.
Steps:
1. Observe the color: normal urine is pale yellow to amber.
Dark yellow may suggest dehydration.
Red or brown may indicate blood or myoglobin.
2. Check clarity: normal is clear.
Cloudy may suggest infection, pus, or crystals.
3. Note odor: a foul smell can indicate infection.
πΉ STEP 3: CHEMICAL ANALYSIS (USING DIPSTICK)
Explanation:
A urine dipstick has multiple reagent pads that change color based on chemical composition.
Steps:
1. Dip the dipstick completely into the urine sample.
2. Remove it immediately and tap off excess urine.
3. Wait the recommended time (usually 30 seconds to 2 minutes) for each test pad.
4. Compare the color of each pad with the manufacturer’s color chart on the strip container.
Parameters typically tested:
pH – Normal: 4.5–8.0 (alkaline or acidic)
Specific Gravity – Indicates urine concentration.
Protein – Detects kidney damage or infections.
Glucose – Suggests diabetes.
Ketones – Seen in diabetes or starvation.
Blood – Suggests infection, trauma, or kidney stones.
Leukocytes – Indicates white blood cells (infection).
Nitrites – Suggests bacterial infection.
Urobilinogen/Bilirubin – Liver function.
πΉ STEP 4: MICROSCOPIC EXAMINATION
Explanation:
Done to detect cells, crystals, casts, and organisms under a microscope.
Steps:
1. Pour about 10 mL of urine into a test tube.
2. Centrifuge the sample at about 1500–3000 rpm for 5 minutes.
3. Discard the supernatant, leaving a small drop of sediment.
4. Place 1 drop of sediment on a glass slide, cover with a coverslip.
5. Examine under a microscope (low power first, then high power).
Look for:
RBCs (Red Blood Cells) – bleeding or trauma.
WBCs (White Blood Cells) – infection.
Casts – formed in kidney tubules (e.g., hyaline, granular, RBC casts).
Crystals – can indicate kidney stones or metabolic disorders.
Bacteria, yeast, parasites – infection.
πΉ STEP 5: DOCUMENTATION & INTERPRETATION
Explanation:
Results must be accurately recorded and interpreted by a healthcare professional.
Steps:
1. Record findings from physical, chemical, and microscopic exams.
2. Compare with normal reference ranges.
3. Correlate findings with clinical symptoms.
4. Submit the report to the attending physician for diagnosis and treatment planning.
✅ IMPORTANT TIPS:
Use fresh urine samples, preferably within 2 hours.
Avoid cross-contamination.
Always wear gloves and follow standard infection control practices.
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